Facility

The DALSA facility is currently housed by DTU Construct at the DTU Kgs. Lyngby Campus (building 413), and staffed by experts from DTU Bioengineering, DTU Construct, and DTU Electro.

The current setup is focused on supporting interdisciplinary projects within: 

  • Automation technology development, such as methods for connecting robotics units (integration and scheduling) and developing new hardware such as robotics arms and new HT sensor types. 
  •  Cell-free workflow development, e.g. robotic workflows involving DNA and proteins.

The facility does currently NOT support work with Genetically Modified Organisms. 

 

Luckily, much of the equipment in the Proto-DALSA facility has a twin in the GMO certified laboratories at DTU Bioengineering. 

 

The fully developed DALSA facility will allow for both GMO and non-GMO projects. 

 

Access
If you would like to use the facility or run your project using our equipment, please just reach out to our automation scientist Aske Unger (askung@dtu.dk). 

Equipment at DALSA Co-creation Facility

 

1. Spectrophotometers and Plate Readers:

  • Perkin Elmer Victor 3 1420 Mult Label Counter
  • Perkin Elmer Envision 2104 Multilabel Reader
  • Perkin Elmer Multimode Plate Reader
2. Liquid Handling Systems:
  • Agilent G2991A 4200 Tapestation
  • Tecan 189MP/63DWP Carousel
  • Tecan Freedom Evo-2 200 base Liquid Handling System
  • Tecan Freedom Evo-2 200 base Liquid Handling System
  • Tecan Instrument Fluent 780 (Fluent)
  • Mantis Microfluidic liquid dispenser
  • Mantis V3.3 ACC RFID Liquid Handling Unit
  • Hamilton MicroLab Star Liquid Handler
  • Opentrons OT-2 Liquid Handlers
  • Integra Assist Plus Pipetting robot
  • Integra mini 96 Liquid Handler
  • Integra VIAFLO 384 Bench Top Pipette.

3. Automated Analysis and Imaging Systems:

  • Perkin Elmer Operetta CLS High Content Analysis System
  • Tecan Spark Cell Imager
  • BMG Labtech Nephel Ostar Instrument for measuring precipitation/solubility
  • Jess Protein Simple Automated western blotting system
  • Thermo Scientific Multidrop Combi Microplate Dispenser

4. Sample Preparation and Extraction:

  • Qiagen QIAsymphony SP Sample preparation system for RNA extraction

5. High Throughput SPR System:

  • GE Healthcare Biacore 8K Goldseal High Throughput SPR System (2018)
6. Real-time PCR System:
  • Applied Biosystems ViiA 7 Real-time PCR system with twister, II plate stacker, 2 array card blocks, and 3 spare well heads
7. Meso Scale Discovery (MSD) System:
  • MSD 1201 Meso Sector S 600
8. Robotic arms;
  • 3x Techman cobots: TM5-900
  • 2 x AB Twister II arm and plate stackers
  • 1x Home-built SCARA (Student Project)

Equipment at DALSA GMO Lab

 
1. Picking and plating
  • Singer Rotor + plate imager (plating of cells)
  • Qpix2 colony picker (cherry picking + reform plates)
2. Growth monitoring
  • 3x BioLector II (OD based growth curves)
  • 4x Reshape imagers (time-lapse of plates and Petri dishes)
  • Growth profiler (image based from top)

+ 64 fermentor setup in the platform for high quality physiology data at DTU Bioengineering

 
3. Liquid handling systems
  • 2x FlowbotOne
  • 3x Opentrons OT-2
  • Hamilton MicroLab Star with automated incubator (for fermentation test)
  • Tecan Fluent 780 incl. carousel and plate hotel